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Theranostics. 2013 May 5;3(6):395-408. doi: 10.7150/thno.5935. Print 2013.

Molecular beacons of xeno-nucleic acid for detecting nucleic acid.

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1
College of Public Health, Nantong University, China.

Abstract

Molecular beacons (MBs) of DNA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes the different unnatural sugar backbones of oligonucleotides combined with fluorophores that have been employed to sense DNA/RNA. With different probes, we epitomize the fundamental understanding of driving forces and these recognition processes. Moreover, we will introduce a few novel and attractive emerging applications and discuss their advantages and disadvantages. We also highlight several perspective probes in the application of cancer therapeutics.

KEYWORDS:

Diagnostics.; Molecular beacon; Nucleic acid; Xeno-nucleic acid

PMID:
23781286
PMCID:
PMC3677410
DOI:
10.7150/thno.5935
[Indexed for MEDLINE]
Free PMC Article
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