Format

Send to

Choose Destination
Anticancer Res. 2013 Jul;33(7):2875-9.

Induction of nitric oxide production in RAW264.7 cells under serum-free conditions by O-antigen polysaccharide of lipopolysaccharide.

Author information

1
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kagawa, Japan.

Abstract

BACKGROUND:

Lipopolysaccharide derived from Pantoea agglomerans (LPSp) mainly consists of two aggregates, the high-molecular aggregate (HMM-LPSp) and the low-molecular aggregate (LMM-LPSp). The structural differences between HMM-LPSp and LMM-LPSp seem to depend on the length of the O-antigen polysaccharide because the lipid A regions of the two fractions are quite similar. In this study, we examined the biological activity of LPSp focusing on the O-antigen polysaccharide using HMM-LPSp and LMM-LPSp under serum-free conditions.

MATERIALS AND METHODS:

The binding of LPSp to RAW264.7 cells under serum-free conditions was analyzed by flow cytometry using LPSp-conjugated fluorescein isothiocyanate (FITC-LPSp). The biological activities of HMM-LPSp and LMM-LPSp under serum-free conditions were evaluated by the nitric oxide production.

RESULTS:

FITC-LPSp showed higher fluorescence intensity under serum-free than serum-containing conditions. HMM-LPSp induced higher nitric oxide production than LMM-LPSp under serum-free conditions.

CONCLUSION:

The present study indicates that the reactivity of LPSp is affected by the O-antigen polysaccharide under serum-free conditions.

KEYWORDS:

HMM; LMM; LPS; LPSp; O-antigen polysaccharide; Pantoea agglomerans; serum-free

PMID:
23780973
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center