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PLoS One. 2013 Jun 11;8(6):e66042. doi: 10.1371/journal.pone.0066042. Print 2013.

Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci.

Author information

1
INRA, UMR1319 MICALIS, Jouy en Josas, France ; AgroParistech, UMR MICALIS, Jouy en Josas, France. mesabba@umich.edu

Abstract

We described a quorum-sensing mechanism in the streptococci genus involving a short hydrophobic peptide (SHP), which acts as a pheromone, and a transcriptional regulator belonging to the Rgg family. The shp/rgg genes, found in nearly all streptococcal genomes and in several copies in some, have been classified into three groups. We used a genetic approach to evaluate the functionality of the SHP/Rgg quorum-sensing mechanism, encoded by three selected shp/rgg loci, in pathogenic and non-pathogenic streptococci. We characterized the mature form of each SHP pheromone by mass-spectrometry. We produced synthetic peptides corresponding to these mature forms, and used them to study functional complementation and cross-talk between these different SHP/Rgg systems. We demonstrate that a SHP pheromone of one system can influence the activity of a different system. Interestingly, this does not seem to be dependent on the SHP/Rgg group and cross-talk between pathogenic and non-pathogenic streptococci is observed.

PMID:
23776602
PMCID:
PMC3679016
DOI:
10.1371/journal.pone.0066042
[Indexed for MEDLINE]
Free PMC Article
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