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J Biomed Mater Res A. 2014 Jun;102(6):1726-36. doi: 10.1002/jbm.a.34839. Epub 2013 Jun 24.

Effects of mesoporous SiO2 , Fe3 O4 , and TiO2 nanoparticles on the biological functions of endothelial cells in vitro.

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Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China.


To comparatively investigate the cytotoxicities of nanomaterials in circulation, in this study, three different types of nanoparticles (NPs; mesoporous SiO2, Fe3O4, and TiO2) with diameters of around 100 nm were synthesized. The morphologies, crystalline phases, and zeta potentials of those NPs were characterized by scanning electron microscopy, X-ray diffraction and zeta potential measurement, respectively. Then, we investigated the influences of different NPs on the biological functions of endothelial cells, in particular of the organelle of cells. The results indicated that different types of NPs had cytotoxic effects in a dose- and time-dependent manner, and there was no significant difference in cytotoxicity between SiO2 and Fe3O4 at concentrations <0.20 mg/mL. The shape and surface charges of NPs greatly affected cellular internalization. We found that cytoskeleton and integrity of cells were destroyed by different NPs. Additionally, the production of reactive oxygen species damaged the mitochondria of cells, in turn leading to cells apoptosis and death.


cytotoxicity; endothelial cells; mitochondrion; nanoparticles; reactive oxygen species

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