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Proteomics Clin Appl. 2013 Oct;7(9-10):648-56. doi: 10.1002/prca.201200137. Epub 2013 Sep 17.

Serum Mac-2 binding protein levels as a novel diagnostic biomarker for prediction of disease severity and nonalcoholic steatohepatitis.

Author information

1
Department of Molecular Biochemistry & Clinical Investigation, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
2
Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
3
Department of Hepatology, Graduate School of Medicine, Osaka City University, Abeno-ku, Osaka, Japan.
4
Department of Gastroenterology, Ikeda Municipal Hospital, Ikeda, Osaka, Japan.
5
Department of Gastroenterology, Otemae Hospital, Osaka, Japan.
6
Department of Surgery, Matsumoto Hospital, Kakogawa, Hyogo, Japan.
7
Immuno-Biological Laboratories, Fujioka, Gunma, Japan.

Abstract

PURPOSE:

Mac-2 binding protein (Mac-2 bp) is one of the major fucosylated glycoproteins, which we identified with glycol-proteomic analyses. We previously reported that fucosylated glycoproteins are secreted into bile, but scarcely secreted into sera in normal liver and hypothesized that the fucosylation-based sorting machinery would be disrupted in ballooning hepatocytes due to the loss of cellular polarity. In the present study, we investigated the availability of Mac-2 bp for differential diagnosis of nonalcoholic steatohepatitis (NASH) from nonalcoholic fatty liver disease (NAFLD) as a biomarker.

EXPERIMENTAL DESIGN:

Serum Mac-2 bp levels were determined with our developed ELISA kit. Our cohort of 127 patients with NAFLD had liver biopsy to make a histological diagnosis of NASH and simple fatty liver.

RESULTS:

Mac-2 bp levels were significantly elevated in NASH patients compared with non-NASH (simple steatosis) patients (2.132 ± 1.237 vs. 1.103 ± 0.500 μg/mL, p < 0.01). The area under the receiver-operating characteristic curve for predicting NASH by Mac-2 bp was 0.816. Moreover, multivariate logistic regression analyses showed Mac-2 bp levels could predict the fibrosis stage and the presence of ballooning hepatocytes in NAFLD patients.

CONCLUSIONS AND CLINICAL RELEVANCE:

These results support the potential usefulness of measuring Mac-2 bp levels in clinical practice as a biomarker for NASH.

KEYWORDS:

Ballooning hepatocyte; Liver fibrosis; Mac-2bp/90K; N-glycan; NASH/NAFLD

PMID:
23775887
DOI:
10.1002/prca.201200137
[Indexed for MEDLINE]

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