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Nat Commun. 2013;4:1982. doi: 10.1038/ncomms2982.

Structure and function of Parkin E3 ubiquitin ligase reveals aspects of RING and HECT ligases.

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  • 1Elan Pharmaceuticals, 180 Oyster Point Boulevard, South San Francisco, California 94080, USA.

Abstract

Parkin is a RING-between-RING E3 ligase that functions in the covalent attachment of ubiquitin to specific substrates, and mutations in Parkin are linked to Parkinson's disease, cancer and mycobacterial infection. The RING-between-RING family of E3 ligases are suggested to function with a canonical RING domain and a catalytic cysteine residue usually restricted to HECT E3 ligases, thus termed 'RING/HECT hybrid' enzymes. Here we present the 1.58 Å structure of Parkin-R0RBR, revealing the fold architecture for the four RING domains, and several unpredicted interfaces. Examination of the Parkin active site suggests a catalytic network consisting of C431 and H433. In cells, mutation of C431 eliminates Parkin-catalysed degradation of mitochondria, and capture of an ubiquitin oxyester confirms C431 as Parkin's cellular active site. Our data confirm that Parkin is a RING/HECT hybrid, and provide the first crystal structure of an RING-between-RING E3 ligase at atomic resolution, providing insight into this disease-related protein.

PMID:
23770887
PMCID:
PMC3709503
DOI:
10.1038/ncomms2982
[PubMed - indexed for MEDLINE]
Free PMC Article
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