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Nat Methods. 2013 Aug;10(8):751-4. doi: 10.1038/nmeth.2521. Epub 2013 Jun 16.

Near-infrared fluorescent proteins for multicolor in vivo imaging.

Author information

1
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA.

Abstract

Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs--iRFP670, iRFP682, iRFP702 and iRFP720--from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.

PMID:
23770755
PMCID:
PMC3737237
DOI:
10.1038/nmeth.2521
[Indexed for MEDLINE]
Free PMC Article

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