Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2013 Aug;10(8):747-50. doi: 10.1038/nmeth.2522. Epub 2013 Jun 16.

Genome-wide profiling of human cap-independent translation-enhancing elements.

Author information

1
Center for Evolutionary Medicine and Informatics in the Biodesign Institute, Arizona State University, Tempe, Arizona, USA.

Abstract

We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.

PMID:
23770754
PMCID:
PMC3731418
DOI:
10.1038/nmeth.2522
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center