Somatic mutation frequencies observed in exomes from 3,083 tumor-normal pairs. Each dot corresponds to a tumor-normal pair, with vertical position indicating the total frequency of somatic mutations in the exome. Tumor types are ordered by their median somatic mutation frequency, with the lowest frequencies (left) found in hematological and pediatric tumors, and the highest (right) in tumors induced by carcinogens such as tobacco smoke and UV light. Mutation frequencies vary more than 1000-fold between lowest and highest mutation rates across cancer and also within several tumor types. The lower panel shows the relative proportions of the six different possible base-pair substitutions, as indicated in the legend on the left. (See also .)

Radial spectrum plot of the 2,892 tumor samples having at least 10 coding mutations. The angular space is compartmentalized into the six different factors discovered by NMF (see Methods). The distance from the center represents the total mutation frequency. Different tumor types segregate into different compartments based on their mutation spectra. Notable examples are: lung adenocarcinoma and lung squamous carcinoma (red; 2 o’clock position), melanoma (black; 12 o’clock position), stomach, esophageal and colorectal cancer (various shades of green; 8 o’clock position), samples harboring mutations of the HPV or APOBEC signature (bladder, cervical and head and neck cancer, marked in yellow, orange, and blue respectively; 10 o’clock position), and AML and CLL samples sharing the Tp*A→T signature, 4 o’clock position. (See also .)

Mutation rate varies widely across the genome and correlates with DNA replication time and expression level. (a,b) Mutation rate, replication time, and expression level plotted across selected regions of the genome. Red shows total noncoding mutation rate calculated from whole-genome sequences of 126 samples (excluding exons). Blue shows replication time. Green shows average expression level across 91 cell lines in the Cancer Cell Line Encyclopedia (CCLE), determined by RNA sequencing. (Note that low expression is at the top of the scale and high expression at the bottom, in order to emphasize the mutual correlations with the other variables). Shown are (a) entire chromosome 14 and (b) portions of chromosomes 1 and 8, with the locations of two specific loci: a cluster of 16 olfactory receptors on chr1 and the gene CSMD3 on chr8. These two loci have very high mutation rates, late replication times, and low expression levels. (The local mutation rate at CSMD3 is even higher than predicted from replication time and expression, suggesting contributions from additional factors, perhaps locally increased DNA breakage: the locus is a known fragile site). (c,d) Correlation of mutation rate with expression level and replication time, for all 100 Kb windows across the genome. (e,f) Cumulative distribution of various gene families as a function of expression level and replication time. Olfactory receptor genes, genes encoding long proteins (>4,000aa) and genes spanning large genomic loci (>1Mb) are significantly enriched towards lower expression and later replication. In contrast, known cancer genes (as listed in the Cancer Gene Census) trend toward slightly higher expression and earlier replication. (See also and , , .)