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J Biol Chem. 2013 Aug 9;288(32):23586-96. doi: 10.1074/jbc.M113.489302. Epub 2013 Jun 13.

Microvesicle-mediated transfer of microRNA-150 from monocytes to endothelial cells promotes angiogenesis.

Author information

1
Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, China.

Abstract

Recent studies by our group and others show that microRNAs can be actively secreted into the extracellular environment through microvesicles (MVs) and function as secretory signaling molecules that influence the recipient cell phenotypes. Here we investigate the role of monocyte-secreted miR-150 in promoting the capillary tube formation of endothelial cells and in enhancing angiogenesis. In vitro capillary tube formation and in vivo angiogenesis assays showed that monocyte-derived MVs have strong pro-angiogenic activities. By depleting miR-150 from monocytic MVs and increasing miR-150 in MVs derived from cells that normally contain low levels of miR-150, we further demonstrated that the miR-150 content accounted for the pro-angiogenic activity of monocytic MVs in these assays. Using tumor-implanted mice and ob/ob mice as models, we revealed that miR-150 secretion, which is increased for diseases such as cancers and diabetes, significantly promotes angiogenesis. The delivery of anti-miR-150 antisense oligonucleotides into tumor-implanted mice and ob/ob mice via MVs, however, strongly reduced angiogenesis in both types of mice. Our results collectively demonstrate that secretion of miR-150 via MVs can promote angiogenesis in vitro and in vivo, and we also present a novel microRNA-based therapeutic approach for disease treatment.

KEYWORDS:

Angiogenesis; Atherosclerosis; Cancer; Diabetes; Endothelial Cell; MicroRNA; Microvesicle; Monocyte; miR-150

PMID:
23766514
PMCID:
PMC3949332
DOI:
10.1074/jbc.M113.489302
[Indexed for MEDLINE]
Free PMC Article
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