Format

Send to

Choose Destination
Biomol NMR Assign. 2014 Oct;8(2):225-8. doi: 10.1007/s12104-013-9488-0. Epub 2013 Jun 14.

Backbone and side-chain assignments of an effector membrane localization domain from Vibrio vulnificus MARTX toxin.

Author information

1
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Abstract

(1)H, (13)C, and (15)N chemical shift assignments are presented for the isolated four-helical bundle membrane localization domain from the domain of unknown function 5 (DUF5) effector (MLD(VvDUF5)) of the MARTX toxin from Vibrio vulnificus in its solution state. We have assigned 97% of all backbone and side-chain carbon atoms, including 96% of all backbone residues. Secondary chemical shift analysis using TALOS+ demonstrates four helices that align with those predicted by structure homology modeling using the MLDs of Pasteurella multocida toxin (PMT) and the clostridial TcdB and TcsL toxins as templates. Future studies will be towards solving the structure and determining the dynamics in the solution state.

PMID:
23765285
PMCID:
PMC3859858
DOI:
10.1007/s12104-013-9488-0
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center