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PLoS One. 2013 Jun 6;8(6):e65346. doi: 10.1371/journal.pone.0065346. Print 2013.

Design of a modular protein-based MRI contrast agent for targeted application.

Author information

1
Research Group Structural and Medicinal Biochemistry, Centre for Medical Biotechnology, University of Duisburg-Essen, Essen, Germany.

Abstract

Magnetic resonance imaging (MRI) offers a non-radioactive alternative for the non-invasive detection of tumours. Low molecular weight MRI contrast agents currently in clinical use suffer either from a lack of specificity for tumour tissue or from low relaxivity and thus low contrast amplification. In this study, we present the newly designed two domain fusion protein Zarvin, which is able to bind to therapeutic IgG antibodies suitable for targeting, while facilitating contrast enhancement through high affinity binding sites for Gd(3+). We show that the Zarvin fold is stable under serum conditions, specifically targets a cancer cell-line when bound to the Cetuximab IgG, and allows for imaging with high relaxivity, a property that would be advantageous for the detection of small tumours and metastases at 1.5 or 3 T.

PMID:
23762349
PMCID:
PMC3675113
DOI:
10.1371/journal.pone.0065346
[Indexed for MEDLINE]
Free PMC Article

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