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PLoS One. 2013 Jun 6;8(6):e65284. doi: 10.1371/journal.pone.0065284. Print 2013.

Association of eosinophilic inflammation with FKBP51 expression in sputum cells in asthma.

Author information

1
Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Abstract

BACKGROUND:

Airway eosinophilia is a predictor of steroid responsiveness in steroid-naïve asthma. However, the relationship between airway eosinophilia and the expression of FK506-binding protein 51 (FKBP51), a glucocorticoid receptor co-chaperone that plays a role in steroid insensitivity in asthma, remains unknown.

OBJECTIVE:

To evaluate the relationship between eosinophilic inflammation and FKBP51 expression in sputum cells in asthma.

METHODS:

The FKBP51 mRNA levels in sputum cells from steroid-naïve patients with asthma (n = 31) and stable asthmatic patients on inhaled corticosteroid (ICS) (n = 28) were cross-sectionally examined using real-time PCR. Associations between FKBP51 levels and clinical indices were analyzed.

RESULTS:

In steroid-naïve patients, the FKBP51 levels were negatively correlated with eosinophil proportions in blood (r = -0.52) and sputum (r = -0.57), and exhaled nitric oxide levels (r = -0.42) (all p<0.05). No such associations were observed in patients on ICS. In steroid-naïve patients, improvement in forced expiratory volume in one second after ICS initiation was correlated with baseline eosinophil proportions in blood (r = 0.74) and sputum (r = 0.76) and negatively correlated with FKBP51 levels (r = -0.73) (all p<0.0001) (n = 20). Lastly, the FKBP51 levels were the lowest in steroid-naïve asthmatic patients, followed by mild to moderate persistent asthmatic patients on ICS, and the highest in severe persistent asthmatic patients on ICS (p<0.0001).

CONCLUSIONS:

Lower FKBP51 expression in sputum cells may reflect eosinophilic inflammation and glucocorticoid responsiveness in steroid-naïve asthmatic patients.

PMID:
23762334
PMCID:
PMC3675168
DOI:
10.1371/journal.pone.0065284
[Indexed for MEDLINE]
Free PMC Article

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