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Biol Direct. 2013 Jun 7;8:12. doi: 10.1186/1745-6150-8-12.

Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3'-untranslated regions.

Author information

1
Department of Genome and Gene Expression Data Analysis, Bioinformatics Institute, 30 Biopolis str #07-01, Singapore.

Abstract

Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their corresponding probes is detectable on the expression microarrays. Exosomal mRNA fragmentation is characterized with a specific structural pattern. The closer to the 3'-end of the transcript the fragments are localized, the larger fraction among the secreted RNAs they constitute. Since the 3'-ends of transcripts contain elements conferring subcellular localization of mRNA and are rich in miRNA-binding sites, exosomal RNA may act as competing RNA to regulate stability, localization and translation activity of mRNAs in recipient cells.

REVIEWERS:

This article was reviewed by Neil Smalheiser and Sandor Pongor.

PMID:
23758897
PMCID:
PMC3732077
DOI:
10.1186/1745-6150-8-12
[Indexed for MEDLINE]
Free PMC Article

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