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Reprod Sci. 2014 Feb;21(2):204-14. doi: 10.1177/1933719113492210. Epub 2013 Jun 11.

Aberrant glycosylation of plasma proteins in severe preeclampsia promotes monocyte adhesion.

Author information

1
1Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Madigan Army Medical Center, Tacoma, WA, USA.

Abstract

Glycosylation of plasma proteins increases during pregnancy. Our objectives were to investigate an anti-inflammatory role of these proteins in normal pregnancies and determine whether aberrant protein glycosylation promotes monocyte adhesion in preeclampsia. Plasma was prospectively collected from nonpregnant controls and nulliparous patients in all 3 trimesters. Patients were divided into cohorts based on the applicable postpartum diagnosis. U937 monocytes were preconditioned with enzymatically deglycosylated plasma, and monocyte adhesion to endothelial cell monolayers was quantified by spectrophotometry. Plasma from nonpregnant controls, first trimester normotensives, and first trimester patients with mild preeclampsia inhibited monocyte-endothelial cell adhesion (P < .05), but plasma from first trimester patients with severe preeclampsia and second and third trimester normotensives did not. Deglycosylating plasma proteins significantly increased adhesion in all the cohorts. These results support a role of plasma glycoprotein interaction in monocyte-endothelial cell adhesion and could suggest a novel therapeutic target for severe preeclampsia.

KEYWORDS:

adhesion; glycosylation; monocyte; pregnancy; severe preeclampsia

PMID:
23757314
PMCID:
PMC3879990
DOI:
10.1177/1933719113492210
[Indexed for MEDLINE]
Free PMC Article

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