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Environ Microbiol Rep. 2012 Feb;4(1):36-41. doi: 10.1111/j.1758-2229.2011.00275.x. Epub 2011 Aug 22.

Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities.

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1
Lawrence Berkeley National Laboratory, Life Sciences Division, Berkeley, CA, USA. Department of Earth and Planetary Science Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, CA, USA. Centre for Microscopy and Microanalysis (CMM), The University of Queensland, St. Lucia, Queensland, Australia.

Abstract

Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.

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