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Obesity (Silver Spring). 2013 Jul;21(7):1380-8. doi: 10.1002/oby.20159. Epub 2013 Jun 11.

Activation of adipose tissue macrophages in obese mice does not require lymphocytes.

Author information

1
Division of Endocrinology, Diabetes & Metabolism, Children's Hospital Los Angeles, California, USA.

Abstract

OBJECTIVE:

Macrophages which infiltrate adipose tissue and secrete proinflammatory cytokines may be responsible for obesity-induced insulin resistance. However, the reason why macrophages migrate into adipose tissue and become activated remains unknown though some studies suggest that this may be regulated by T and B lymphocytes. In this study, it has been tested whether T and B lymphocytes and natural killer (NK) cells were necessary for the obesity-induced activation of macrophages in adipose tissue.

DESIGN AND METHODS:

NOD/SCID/IL2-receptor gamma-chain knockout (NSG) mice, which lack mature T and B lymphocytes and NK cells, were made obese by selectively reducing litters and weaning onto a high-fat diet. Mice were then maintained on the diet for 10-11 weeks.

RESULTS:

Adipose tissue from obese NSG mice had more activated macrophages than nonobese mice. These macrophages were found in "crown-like structures" surrounding adipocytes, and expressed higher levels of the inflammatory cytokine TNF-α. However, obesity did not impair glucose tolerance in the NSG mice.

CONCLUSIONS:

These studies demonstrated that T and B lymphocytes and NK cells are not necessary for adipose tissue macrophage activation in obese mice. T and B lymphocytes and/or NK cells may be necessary for the development of obesity-induced impaired glucose tolerance.

PMID:
23754826
PMCID:
PMC3742678
DOI:
10.1002/oby.20159
[Indexed for MEDLINE]
Free PMC Article

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