Format

Send to

Choose Destination
See comment in PubMed Commons below
Rapid Commun Mass Spectrom. 2013 Jul 30;27(14):1660-8. doi: 10.1002/rcm.6610.

Ultraviolet laser-induced cross-linking in peptides.

Author information

1
Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario di Monte S.Angelo, Napoli, Italy.

Abstract

RATIONALE:

The aim of this study was to demonstrate, and to characterize by high-resolution mass spectrometry that it is possible to preferentially induce covalent cross-links in peptides by using high-energy femtosecond ultraviolet (UV) laser pulses. The cross-link is readily formed only when aromatic amino acids are present in the peptide sequence.

METHODS:

Three peptides, xenopsin, angiotensin I, and interleukin, individually or in combination, were exposed to high-energy femtosecond UV laser pulses, either alone or in the presence of spin trapping molecules, the reaction products being characterized by high resolution mass spectrometry.

RESULTS:

High-resolution mass spectrometry and spin trapping strategies showed that cross-linking occurs readily, proceeds via a radical mechanism, and is the highly dominant reaction, proceeding without causing significant photo-damage in the investigated range of experimental parameters.

CONCLUSIONS:

High-energy femtosecond UV laser pulses can be used to induce covalent cross-links between aromatic amino acids in peptides, overcoming photo-oxidation processes, that predominate as the mean laser pulse intensity approaches illumination conditions achievable with conventional UV light sources.

PMID:
23754800
PMCID:
PMC3882510
DOI:
10.1002/rcm.6610
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Support Center