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Methods Mol Biol. 2013;1010:155-76. doi: 10.1007/978-1-62703-411-1_11.

The CGG repeat and the FMR1 gene.

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  • 1Department of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands.

Abstract

This chapter intends to provide the different DNA and protein methods for analysis of the repeat in the FMR1 gene, both in humans and in mouse models. The methods to determine the CGG repeat size include Southern blot analysis, polymerase chain reaction (PCR), and methylation status analysis, including bisulfite treatment. The methods to determine expression include analysis of RNA levels and protein levels.

PMID:
23754225
DOI:
10.1007/978-1-62703-411-1_11
[PubMed - indexed for MEDLINE]
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