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Nat Methods. 2013 Jul;10(7):634-7. doi: 10.1038/nmeth.2518. Epub 2013 Jun 9.

Integrated proteomic analysis of post-translational modifications by serial enrichment.

Author information

1
The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. pmertins@broadinstitute.org

Abstract

We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.

PMID:
23749302
PMCID:
PMC3943163
DOI:
10.1038/nmeth.2518
[Indexed for MEDLINE]
Free PMC Article

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