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J Biotechnol. 2013 Jul 20;166(4):187-91. doi: 10.1016/j.jbiotec.2013.05.013. Epub 2013 Jun 7.

Extension of Sphingobium sp. BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering.

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1
Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing, PR China.

Abstract

The gene cassette encoding for TftAB and TftCD proteins was integrated into the 16srDNA gene of the γ-hexachlorocyclohexane (γ-HCH) mineralizing strain Sphingobium sp. BHC-A by homologous recombination. The recombinant γ-HCH mineralizing strain may degrade 2,4,5-trichlorophenoxyacetic acid at a rate of 250nmol mg [protein](-1)h(-1), and the generated intermediate 2,5-dichlorohydroquinone may be further mineralized through γ-HCH downstream degradation pathway.

KEYWORDS:

2,4,5-Trichlorophenoxyacetic acid; 2,5-Dichlorohydroquinone; Homologous recombination; Mineralization; γ-Hexachlorocyclohexane

PMID:
23747683
DOI:
10.1016/j.jbiotec.2013.05.013
[Indexed for MEDLINE]
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