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Anal Biochem. 2013 Sep 15;440(2):120-2. doi: 10.1016/j.ab.2013.05.032. Epub 2013 Jun 6.

Real-time monitoring of disintegration activity of catalytic core domain of HIV-1 integrase using molecular beacon.

Author information

1
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, People's Republic of China.

Abstract

HIV-1 integrase, an essential enzyme for retroviral replication, is a validated target for anti-HIV therapy development. The catalytic core domain of integrase (IN-CCD) is capable of catalyzing disintegration reaction. In this work, a hairpin-shaped disintegration substrate was designed and validated by enzyme-linked immunosorbent assay; a molecular beacon-based assay was developed for disintegration reaction of IN-CCD. Results showed that the disintegration substrate could be recognized and catalyzed by IN-CCD, and the disintegration reaction can be monitored according to the increase of fluorescent signal. The assay can be applied to real-time detection of disintegration with advantages of simplicity, high sensitivity, and excellent specificity.

KEYWORDS:

Disintegration; HIV-1; IN-CCD; Integrase; Molecular beacon

PMID:
23747532
DOI:
10.1016/j.ab.2013.05.032
[Indexed for MEDLINE]
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