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Anal Biochem. 2013 Sep 15;440(2):186-8. doi: 10.1016/j.ab.2013.05.027. Epub 2013 Jun 6.

Stain-Free total protein staining is a superior loading control to β-actin for Western blots.

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1
Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA 95616, USA.

Abstract

Semi-quantification of proteins using Western blots typically involves normalization against housekeeping genes such as β-actin. More recently, Ponceau S and Coomassie blue staining have both been shown to be suitable alternatives to housekeeping genes as loading controls. Stain-Free total protein staining offers the advantage of no staining or destaining steps. Evaluation of the use of Stain-Free staining as an alternative to β-actin or the protein stain Ponceau S showed that Stain-Free staining was superior to β-actin and as good as or better than Ponceau S staining as a loading control for Western blots.

KEYWORDS:

Nitrocellulose; Ponceau S; Stain Free; Western blotting; β-Actin

PMID:
23747530
PMCID:
PMC3809032
DOI:
10.1016/j.ab.2013.05.027
[Indexed for MEDLINE]
Free PMC Article
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