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Curr Biol. 2013 Jun 17;23(12):1046-56. doi: 10.1016/j.cub.2013.04.057. Epub 2013 Jun 6.

Dynamic localization of G-actin during membrane protrusion in neuronal motility.

Author information

1
Department of Cell Biology and Neurology, Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA.

Abstract

BACKGROUND:

Actin-based cell motility is fundamental for development, function, and malignant events in eukaryotic organisms. During neural development, axonal growth cones depend on rapid assembly and disassembly of actin filaments (F-actin) for their guided extension to specific targets for wiring. Monomeric globular actin (G-actin) is the building block for F-actin but is not considered to play a direct role in spatiotemporal control of actin dynamics in cell motility.

RESULTS:

Here we report that a pool of G-actin dynamically localizes to the leading edge of growth cones and neuroblastoma cells to spatially elevate the G-/F-actin ratio that drives membrane protrusion and cell movement. Loss of G-actin localization leads to the cessation and retraction of membrane protrusions. Moreover, G-actin localization occurs asymmetrically in growth cones during attractive turning. Finally, we identify the actin monomer-binding proteins profilin and thymosin β4 as key molecules that localize actin monomers to the leading edge of lamellipodia for their motility.

CONCLUSIONS:

Our results suggest that dynamic localization of G-actin provides a novel mechanism to regulate the spatiotemporal actin dynamics underlying membrane protrusion in cell locomotion and growth cone chemotaxis.

PMID:
23746641
PMCID:
PMC3712510
DOI:
10.1016/j.cub.2013.04.057
[Indexed for MEDLINE]
Free PMC Article

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