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Curr Opin Chem Biol. 2013 Aug;17(4):651-5. doi: 10.1016/j.cbpa.2013.05.014. Epub 2013 Jun 6.

New approaches for sensing metabolites and proteins in live cells using RNA.

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1
Department of Pharmacology, Weill Medical College, Cornell University, 1300 York Avenue, LC 523, New York, NY 10065, USA.

Abstract

Tools to study the abundance, distribution, and flux of intracellular molecules are crucial for understanding cellular signaling and physiology. Although powerful, the current FRET-based technology for imaging cellular metabolites is not easily generalizable. Thus, new platforms for generating genetically encoded sensors are needed. We recently developed a new class of biosensors on the basis of Spinach, an RNA mimic of GFP. In this case, RNA aptamers against a target ligand are modularly fused to Spinach that substantially induce Spinach fluorescence in the presence of ligand. We have used this approach to detect metabolites and proteins both in vitro and in living bacteria, thus providing an alternative to FRET-based sensors and a generalizable approach for generating fluorescent sensors to any ligand of interest.

PMID:
23746618
PMCID:
PMC3742595
DOI:
10.1016/j.cbpa.2013.05.014
[Indexed for MEDLINE]
Free PMC Article
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