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J Biosci Bioeng. 2013 Nov;116(5):620-3. doi: 10.1016/j.jbiosc.2013.05.006. Epub 2013 Jun 4.

Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging.

Author information

1
Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. Electronic address: tanaka.torahiko@nihon-u.ac.jp.

Abstract

A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.

KEYWORDS:

Adipose differentiation-related protein; Hepatitis C virus; Lipid droplets; Nonstructural protein 4B; Time-lapse imaging

PMID:
23742748
DOI:
10.1016/j.jbiosc.2013.05.006
[Indexed for MEDLINE]
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