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Clin Vaccine Immunol. 2013 Aug;20(8):1329-32. doi: 10.1128/CVI.00153-13. Epub 2013 Jun 5.

Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination.

Author information

  • 1Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, the Netherlands. m.scherpenisse@amc.uva.nl

Abstract

We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA.

PMID:
23740920
PMCID:
PMC3754499
DOI:
10.1128/CVI.00153-13
[PubMed - indexed for MEDLINE]
Free PMC Article
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