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Proteomics. 2013 Aug;13(16):2386-97. doi: 10.1002/pmic.201300022. Epub 2013 Jul 11.

A tool to evaluate correspondence between extraction ion chromatographic peaks and peptide-spectrum matches in shotgun proteomics experiments.

Author information

1
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA. ruse@cshl.edu

Abstract

Chromatographed peptide signals form the basis of further data processing that eventually results in functional information derived from data-dependent bottom-up proteomics assays. We seek to rank LC/MS parent ions by the quality of their extracted ion chromatograms. Ranked extracted ion chromatograms act as an intuitive physical/chemical preselection filter to improve the quality of MS/MS fragment scans submitted for database search. We identify more than 4900 proteins when considering detector shifts of less than 7 ppm. High quality parent ions for which the database search yields no hits become candidates for subsequent unrestricted analysis for PTMs. Following this rational approach, we prioritize identification of more than 5000 spectrum matches from modified peptides and confirmed the presence of acetylaldehyde-modified His/Lys. We present a logical workflow that scores data-dependent selected ion chromatograms and leverage information about semianalytical LC/LC dimension prior to MS. Our method can be successfully used to identify unexpected modifications in peptides with excellent chromatography characteristics, independent of fragmentation pattern and activation methods. We illustrate analysis of ion chromatograms detected in two different modes by RF linear ion trap and electrostatic field orbitrap.

KEYWORDS:

Accurate mass measurement; Fast chromatogram inversion; Multidimensional chromatography; Statistical ranking; Technology

PMID:
23733317
PMCID:
PMC3994887
DOI:
10.1002/pmic.201300022
[Indexed for MEDLINE]
Free PMC Article

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