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Curr Opin Chem Biol. 2013 Aug;17(4):663-71. doi: 10.1016/j.cbpa.2013.04.027. Epub 2013 May 31.

Visualizing molecular diffusion through passive permeability barriers in cells: conventional and novel approaches.

Author information

1
Department of Cell Biology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University, United States. ylin54@jhmi.edu

Abstract

Diffusion barriers are universal solutions for cells to achieve distinct organizations, compositions, and activities within a limited space. The influence of diffusion barriers on the spatiotemporal dynamics of signaling molecules often determines cellular physiology and functions. Over the years, the passive permeability barriers in various subcellular locales have been characterized using elaborate analytical techniques. In this review, we will summarize the current state of knowledge on the various passive permeability barriers present in mammalian cells. We will conclude with a description of several conventional techniques and one new approach based on chemically inducible diffusion trap (CIDT) for probing permeable barriers.

PMID:
23731778
PMCID:
PMC3860267
DOI:
10.1016/j.cbpa.2013.04.027
[Indexed for MEDLINE]
Free PMC Article

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