Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Chem Biol. 2013 Aug;9(8):514-20. doi: 10.1038/nchembio.1270. Epub 2013 Jun 2.

Identification of small molecules for human hepatocyte expansion and iPS differentiation.

Author information

1
Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA.

Abstract

Cell-based therapies hold the potential to alleviate the growing burden of liver diseases. Such therapies require human hepatocytes, which, within the stromal context of the liver, are capable of many rounds of replication. However, this ability is lost ex vivo, and human hepatocyte sourcing has limited many fields of research for decades. Here we developed a high-throughput screening platform for primary human hepatocytes to identify small molecules in two different classes that can be used to generate renewable sources of functional human hepatocytes. The first class induced functional proliferation of primary human hepatocytes in vitro. The second class enhanced hepatocyte functions and promoted the differentiation of induced pluripotent stem cell-derived hepatocytes toward a more mature phenotype than what was previously obtainable. The identification of these small molecules can help address a major challenge affecting many facets of liver research and may lead to the development of new therapeutics for liver diseases.

PMID:
23728495
PMCID:
PMC3720805
DOI:
10.1038/nchembio.1270
[Indexed for MEDLINE]
Free PMC Article

Publication types, MeSH terms, Substance, Secondary source ID, Grant support

Publication types

MeSH terms

Substance

Secondary source ID

Grant support

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center