Format

Send to

Choose Destination
See comment in PubMed Commons below
J Vis Exp. 2013 May 26;(75):e3899. doi: 10.3791/3899.

Isolation and genome analysis of single virions using 'single virus genomics'.

Author information

1
Department of Microbial and Environmental Genomics, The J. Craig Venter Institute, USA.

Abstract

Whole genome amplification and sequencing of single microbial cells enables genomic characterization without the need of cultivation (1-3). Viruses, which are ubiquitous and the most numerous entities on our planet (4) and important in all environments (5), have yet to be revealed via similar approaches. Here we describe an approach for isolating and characterizing the genomes of single virions called 'Single Virus Genomics' (SVG). SVG utilizes flow cytometry to isolate individual viruses and whole genome amplification to obtain high molecular weight genomic DNA (gDNA) that can be used in subsequent sequencing reactions.

PMID:
23728084
PMCID:
PMC3719269
DOI:
10.3791/3899
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for MyJove Corporation Icon for PubMed Central
    Loading ...
    Support Center