Aptamer degradation inhibition combined with DNAzyme cascade-based signal amplification for colorimetric detection of proteins

Jishan Li; Yuhua Jia; Jing Zheng; Wenwan Zhong; Guoli Shen; Ronghua Yang; Weihong Tan

doi:10.1039/c3cc42148a

Aptamer degradation inhibition combined with DNAzyme cascade-based signal amplification for colorimetric detection of proteins

Chem Commun (Camb). 2013 Jul 14;49(55):6137-9. doi: 10.1039/c3cc42148a.

Authors

Jishan Li¹, Yuhua Jia, Jing Zheng, Wenwan Zhong, Guoli Shen, Ronghua Yang, Weihong Tan

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China. jishanli@hnu.cn

PMID: 23727767
DOI: 10.1039/c3cc42148a

Abstract

Combining the inhibition of aptamer degradation with DNA Enzyme (DNAzyme) cascade-based signal amplification, a label-free and sensitive colorimetric protein assay strategy was proposed.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Aptamers, Nucleotide / metabolism*
Becaplermin
Biological Assay
Biomarkers / analysis
Colorimetry
DNA, Catalytic / metabolism*
Exodeoxyribonucleases / metabolism*
Nanoparticles
Point-of-Care Systems
Proto-Oncogene Proteins c-sis / analysis*
Proto-Oncogene Proteins c-sis / metabolism

Substances

Aptamers, Nucleotide
Biomarkers
DNA, Catalytic
Proto-Oncogene Proteins c-sis
Becaplermin
Exodeoxyribonucleases
exodeoxyribonuclease I