(A) Representative averaged traces of sIPSCs recorded in an individual WT nRT cell under control conditions (black trace), after 10 min bath application of 100 nM clonazepam (CZP, gray trace), and in CZP with subsequent 10 min bath application of FLZ (CZP+FLZ, red trace), normalized to peak amplitude. (B) sIPSC half width in individual WT nRT cells (n=8) at P20–29 in control conditions (Con), during CZP treatment (CZP), and during subsequent FLZ treatment (CZP+FLZ). Open boxes indicate mean ± SEM during control (black), CZP (gray), and CZP+FLZ conditions (red). (C) Mean + SEM for percentage change in sIPSC half width from control to CZP conditions (gray bar), CZP to CZP+FLZ conditions (red bar), and from control to CZP+FLZ conditions (black bar). (D) Mean + SEM for the within-cell ratio of the percentage change from control to CZP+FLZ conditions compared to the percentage change from CZP alone to CZP+FLZ. (E) Averaged sIPSC traces from control VB cells (black trace) and cells in slices either preincubated in 100 nM clonazepam (CZP, gray trace), or in CZP with subsequent 10 min bath application of FLZ (CZP+FLZ, red trace). Note that FLZ treatment does not reduce sIPSC duration past control values, as compared to nRT cells. (F) Probability distribution for >800 events per group comparing sIPSC half width in control (black line, n=8) and CZP-preincubated cells (gray line, n=11). **p<0.01, ***p<0.001 vs. Control (B, F), ###p<0.001 vs. CZP (B).