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J Microbiol Methods. 2013 Aug;94(2):121-124. doi: 10.1016/j.mimet.2013.05.014. Epub 2013 May 26.

Rapid discrimination of Acinetobacter baumannii international clone II lineage by pyrosequencing SNP analyses of bla(OXA-51-like) genes.

Author information

1
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: m-matsui@nih.go.jp.
2
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
3
Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya Japan.

Abstract

We found that Acinetobacter baumannii international clone II generally possesses unique GTA sequence at nucleotide positions 106-108 in the bla(OXA-51-like) genes. We exploited this to develop an easy and rapid method for discrimination of international clone II from other A. baumannii by employing pyrosequencing analyses of single nucleotide polymorphisms.

KEYWORDS:

Acinetobacter baumannii; Pyrosequence; bla(OXA-51-like)

PMID:
23719027
DOI:
10.1016/j.mimet.2013.05.014
[Indexed for MEDLINE]

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