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J Conserv Dent. 2013 Mar;16(2):135-8. doi: 10.4103/0972-0707.108194.

Effect of collagen cross-linkers on the shear bond strength of a self-etch adhesive system to deep dentin.

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Department of Conservative Dentistry and Endodontics, SRM Dental College, Bharathi Salai, Ramapuram, Chennai, Tamil Nadu, India.



To evaluate the shear bond strength of composite resin to deep dentin, bonded using a self-etch adhesive, after treatment with two collagen cross-linkers at varying time intervals.


Thirty extracted human incisors were sectioned longitudinally into equal mesial and distal halves (n = 60). The proximal deep dentin was exposed and the specimens were divided based on the surface treatment of dentin prior to bonding as follows: Group I (n = 12, control): No prior dentin surface treatment; group II (n = 24): Dentin surface pretreated with 10% sodium ascorbate; and group III (n = 24): Dentin surface pretreated with 6.5% proanthocyanidin. Groups II and III were further divided into two subgroups based on the pre-treatment time of five and 10 min. Shear bond strength of the specimens was tested using universal testing machine and the data were statistically analyzed.


Significantly higher shear bond strength to deep dentin was observed in teeth treated with 10% sodium ascorbate and 6.5% proanthocyanidin compared to control group. No significant difference was observed between 5 min and 10 min pre-treatment times.


Dentin surface pre-treatment with both 10% sodium ascorbate and 6.5% proanthocyanidin resulted in significant improvement in bond strength of self-etch adhesive to deep dentin.


Deep dentin; grape seed extract; self-etch adhesive; shear bond strength; sodium ascorbate

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