Structure of ABCB10 in complex with the nonhydrolysable nucleotide analog AMPPCP, showing that ABCB10 is in an open conformation, even when it is bound to nucleotide analogs. Cartoon representations of the ABCB10/AMPPCP complex monomer (A) and homodimer (B) as seen in the rod-form B crystal structure. The structures have a single monomer in the asymmetric unit, the dimer is generated by a crystallographic twofold.

Comparison of the structures of the ABCB10 homodimer in the absence (apo) and presence of bound nucleotide analogs, with the structures of ceP-gp in the open-inwards conformation [PDB ID code: 4F4C ()] and Sav1866 [PDB ID code 2HYD ()] in the open-outwards conformation. Transporters are viewed perpendicular to their (pseudo) twofold symmetry axes. (B) Alignment of NBDs of the structures shown in A. viewed looking toward the membrane. The ABCB10 monomers (blue/purple and orange/red respectively), nucleotides (green), and the NBD’s C-loop (cyan) are highlighted. The black lines/circles below each NBD pair indicate the translation required to bring the NBDs in the closed conformation for catalysis [the distance is the separation between the nucleotide γ-phosphate (green) and the C-α of the first glycine in the catalytic C loop of the adjacent NBD (cyan)]. An additional rotational component is also required for proper alignment of the NBDs in the closed state. Where trinucleotide is not present in the structure, the position of the γ-phosphate has been inferred by superposition of the AMPPCP complex. The C-terminal extension in the Sav1866 structure has been omitted for clarity.

Interactions of nucleotides with the ABCB10 inward-facing conformation. (A) Schematic representation of NBDs of ABCB10 homodimer in the highest-resolution structure (rod form A) viewed looking from the TMD. AMPPCP (green/orange) are bound to each NBD but do not make inter-NBD interaction with the ABC transporter consensus sequence LSGGQ C-loop motif (cyan). (B) Individual NBD viewed looking from adjacent NBD. NBD (pink) with ABC Transporter nucleotide binding signature motifs colored as in C. TMD (gray) and coupling helices (CH1, orange; CH2^#, purple) are highlighted. Omit F_o − F_c density for AMPPCP/Mg²⁺ (blue mesh) is shown contoured at 3 σ. Dotted box indicates zoomed region in C and D. Detailed view of nucleotide binding site in rod form A/AMPPCP complex (C) and nucleotide-free form (D). Oxygen atoms are colored red, nitrogen atoms blue, phosphate atoms orange and carbon atoms are colored according to the location of the atom: The AMPPCP carbon atoms are shown in green. The conserved NBD sequence motifs are colored Walker A (yellow), Walker B (light blue), A-loop (pale cyan), Gln-loop (red), His-loop (light green), coupling helix 1 (CH1, orange), and coupling helix 2 (CH2, magenta). The C-loop (cyan) is not visible as it is more than 16 Å away from the nucleotide binding site in this conformation. Residues/secondary structure elements marked with a pound symbol (#) denote regions contributed by homodimer partner. The side-chain of His690 is disordered and has not been modeled in rod form A.

ABCB10 has cardiolipin and detergent bound to the transmembrane helices and a portal between helices TMH1 and TMH2, which is open in the rod crystal form and closed in the plate-form crystals. (A) Overview of the ABCB10 structure showing the location of lipid (magenta) and detergent (green) binding sites. (B) Molecular surface representation of the TMD in rod form A crystals, with lipid and detergent molecules shown in magenta and green and the portal between TMH1 and TMH2 indicated with a dotted line. In the rod-form structures TMH1 and TMH2 are loosely packed revealing a 7 Å wide × 30 Å long portal connecting the central cavity of the TMDs with the membrane environment. The portal is occupied by a CDL alkyl chain (magenta). (C) In the plate form crystals TMH1 and TMH2 are packed closer together, with the portal closed. Structures are viewed in the same orientation as in A.

Sequence conservation of residues in ABCB10. Conservation is mapped onto a molecular surface representation of the concave inner cavity (A) and convex outer surface (B) of ABCB10. The molecular surface shown is a composite representing one-half “leaflet” of the transporter dimer, comprising residues from TMH1–TMH3 and TMH6-NBD in one monomer and TMH4/5 from the second monomer (residues 311–424). Residues are colored according to sequence conservation, invariant (dark blue), highly conserved (slate; 2–4 related amino acids), and moderately conserved (pale blue; 3–8 related amino acids). Sequence conservation was calculated based on an alignment of eighty ABCB10 homologs (human to yeast) using the CONSURF server (). The conserved patch in the TMD between TMH2/TMH3B adjacent to the portal region (indicated in red) defines a unique ABCB10 signature sequence. (C and D) Perpendicular views of residues defined by ABCB10 signature sequence (Asn229, Arg232, Asn289, Asp295, and Arg295). All of the side-chains project toward the lumen of the transporter. Side-chains are shown along with a semitransparent molecular surface. (E) Conserved signature sequence defined by residues located in central portion of TMH2 and at the N-terminal end of TMH3B.

Overview of the steps proposed for the transport cycle of ABCB10 and other ABC transporters of the exporter family. The TMDs are colored blue and orange, with their associated NBDs colored purple and red. ATP is shown in green, ADP in gray and the transport substrate in yellow. An asterisk (*) and pound symbol (#) indicate the conformations observed for nucleotide-bound and nucleotide-free ABCB10, respectively.