A global S. cerevisiae small ubiquitin-related modifier (SUMO) system interactome

Mol Syst Biol. 2013 May 28:9:668. doi: 10.1038/msb.2013.23.

Abstract

The small ubiquitin-related modifier (SUMO) system has been implicated in a number of biological functions, yet the individual components of the SUMO machinery involved in each of these activities were largely unknown. Here we report the first global SUMO system interactome. Using affinity purification coupled with mass spectrometry, we identify >450 protein-protein interactions surrounding the SUMO E2, Siz type E3s and SUMO-specific proteases in budding yeast. Exploiting this information-rich resource, we validate several Siz1- and Siz2-specific substrates, identify a nucleoporin required for proper Ulp1 localization, and uncover important new roles for Ubc9 and Ulp2 in the maintenance of ribosomal DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle / genetics
  • Cysteine Endopeptidases / genetics
  • Cysteine Endopeptidases / metabolism
  • DNA, Ribosomal / genetics
  • Endopeptidases / genetics
  • Endopeptidases / metabolism
  • Gene Expression Regulation, Fungal*
  • Nuclear Pore Complex Proteins / genetics
  • Nuclear Pore Complex Proteins / metabolism
  • Protein Interaction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Signal Transduction
  • Small Ubiquitin-Related Modifier Proteins / genetics*
  • Small Ubiquitin-Related Modifier Proteins / metabolism
  • Transcriptome*
  • Ubiquitin / genetics
  • Ubiquitin / metabolism
  • Ubiquitin-Conjugating Enzymes / genetics
  • Ubiquitin-Conjugating Enzymes / metabolism
  • Ubiquitin-Protein Ligases / genetics
  • Ubiquitin-Protein Ligases / metabolism

Substances

  • DNA, Ribosomal
  • Nuclear Pore Complex Proteins
  • Saccharomyces cerevisiae Proteins
  • Siz2 protein, S cerevisiae
  • Small Ubiquitin-Related Modifier Proteins
  • Ubiquitin
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligases
  • Endopeptidases
  • ULP2 protein, S cerevisiae
  • Cysteine Endopeptidases
  • Ulp1 protease
  • Siz1 protein, S cerevisiae
  • ubiquitin-conjugating enzyme UBC9