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Genetics. 2013 Aug;194(4):1029-35. doi: 10.1534/genetics.113.152710. Epub 2013 May 24.

Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.

Author information

1
Genetics Training Program, University of Wisconsin, Madison, Madison, WI 53706, USA.

Abstract

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

KEYWORDS:

CRISPR RNA; Cas9; Drosophila; genome engineering; homologous recombination

PMID:
23709638
PMCID:
PMC3730909
DOI:
10.1534/genetics.113.152710
[Indexed for MEDLINE]
Free PMC Article

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