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Oncogene. 2014 Apr 24;33(17):2245-54. doi: 10.1038/onc.2013.167. Epub 2013 May 27.

PIK3CA and APC mutations are synergistic in the development of intestinal cancers.

Author information

1
Division of Hematology and Oncology, Department of Medicine, University of Wisconsin, Madison, WI, USA.
2
Department of Oncology, University of Wisconsin, Madison, WI, USA.
3
Division of Gastroenterology and Hepatology, Department of Medicine, University of Wisconsin, Madison, WI, USA.
4
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, USA.
5
Promega Corporation, Madison, WI, USA.
6
Department of Pathology and Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN, USA.
7
Department of Radiology, University of Wisconsin, Madison, WI, USA.

Abstract

Human colorectal cancers are known to possess multiple mutations, though how these mutations interact in tumor development and progression has not been fully investigated. We have previously described the FCPIK3ca* murine colon cancer model, which expresses a constitutively activated phosphoinositide-3 kinase (PI3K) in the intestinal epithelium. The expression of this dominantly active form of PI3K results in hyperplasia and invasive mucinous adenocarcinomas. These cancers form via a non-canonical mechanism of tumor initiation that is mediated through activation of PI3K and not through aberrations in WNT signaling. Since the Adenomatous Polyposis Coli (APC) gene is mutated in the majority of human colon cancers and often occurs simultaneously with PIK3CA mutations, we sought to better understand the interaction between APC and PIK3CA mutations in the mammalian intestine. In this study, we have generated mice in which the expression of a constitutively active PI3K and the loss of APC occur simultaneously in the distal small intestine and colon. Here, we demonstrate that expression of a dominant active PI3K synergizes with loss of APC activity resulting in a dramatic change in tumor multiplicity, size, morphology and invasiveness. Activation of the PI3K pathway is not able to directly activate WNT signaling through the nuclear localization of CTNNB1 (β-catenin) in the absence of aberrant WNT signaling. Alterations at the transcriptional level, including increased CCND1, may be the etiology of synergy between these activated pathways.

PMID:
23708654
PMCID:
PMC3883937
DOI:
10.1038/onc.2013.167
[Indexed for MEDLINE]
Free PMC Article

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