Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2013 Jul 12;436(4):565-70. doi: 10.1016/j.bbrc.2013.05.062. Epub 2013 May 23.

Granulocyte colony-stimulating factor induces in vitro lymphangiogenesis.

Author information

1
Department of Internal Medicine, Chonbuk National University Medical School, Jeonju, Republic of Korea.

Abstract

Granulocyte-colony stimulating factor (G-CSF) is reported to induce differentiation in cells of the monocyte lineage and angiogenesis in vascular endothelial cells, but its effects on lymphangiogenesis is uncertain. Here we examined the effects and the mechanisms of G-CSF-induced lymphangiogenesis using human lymphatic endothelial cells (hLECs). Our results showed that G-CSF induced capillary-like tube formation, migration and proliferation of hLECs in a dose- and time-dependent manner and enhanced sprouting of thoracic duct. G-CSF increased phosphorylation of Akt and ERK1/2 in hLECs. Supporting the observations, specific inhibitors of phosphatidylinositol 3'-kinase and MAPK suppressed the G-CSF-induced in vitro lymphangiogenesis and sprouting. Intraperitoneal administration of G-CSF to mice also stimulated peritoneal lymphangiogenesis. These findings suggest that G-CSF is a lymphangiogenic factor.

KEYWORDS:

Granulocyte-colony stimulating factor; Lymphangiogenesis; Lymphangiogenic factor; Lymphatic endothelial cell

PMID:
23707939
DOI:
10.1016/j.bbrc.2013.05.062
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center