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Cell Signal. 2013 Sep;25(9):1748-53. doi: 10.1016/j.cellsig.2013.05.004. Epub 2013 May 22.

Compartmentalized Ras signaling differentially contributes to phenotypic outputs.

Author information

1
Department of Cellular and Molecular Physiology, Institute of Translational Research, University of Liverpool, Liverpool L69 3BX, UK.

Abstract

Ras isoforms are membrane bound proteins that differentially localize to the plasma membrane and subcellular compartments within the cell. Whilst the cell surface is the main site for Ras activity the extent to which intracellular pools contribute to Ras function is debated. We have generated Ras chimeras targeting Ras to the ER, Golgi, mitochondria and endosomes to compare the capacity of each of these locations to support activity equivalent to normal Ras function. We find that all locations are capable of regulating the MAP kinase and Akt pathways. Furthermore, whilst endomembranous Ras pools show location-specific competence to support proliferation and transformation, Golgi-Ras is as potent as N-Ras.

PMID:
23707528
PMCID:
PMC3776226
DOI:
10.1016/j.cellsig.2013.05.004
[Indexed for MEDLINE]
Free PMC Article

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