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J Microbiol Methods. 2013 Aug;94(2):117-20. doi: 10.1016/j.mimet.2013.05.013. Epub 2013 May 24.

A SOE-PCR method of introducing multiple mutations into Mycoplasma gallisepticum neuraminidase.

Author information

  • 1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.

Abstract

A modified splicing with overlap extension PCR (SOE-PCR) was generated to introduce 21 TGA to TGG at Mycoplasma gallisepticum MGA_0329 gene. The recombinant protein was successfully expressed and retained neuraminidase activities, indicating that SOE-PCR is a rapid and highly efficient method of introducing multiple mutations into large M. gallisepticum genes.

KEYWORDS:

M. gallisepticum; MGA_0329 gene; Neuraminidase; SOE-PCR

PMID:
23707236
DOI:
10.1016/j.mimet.2013.05.013
[PubMed - indexed for MEDLINE]
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