Abstract
The synthesis and comparison of activities of 'tag-free' probes with diazirines at various positions are described. Remarkable differences in their effects on P. aeruginosa and on human bronchial epithelial cells were observed, supporting the efforts to isolate and identify receptors for N-acyl homoserine lactones.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Carboxylic Ester Hydrolases / chemistry
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Carboxylic Ester Hydrolases / pharmacology
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Cell Line
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Cycloaddition Reaction
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Diazomethane / chemistry
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Diazomethane / pharmacology*
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Humans
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Molecular Probes / chemistry
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Molecular Probes / pharmacology
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Protein Structure, Tertiary
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Pseudomonas aeruginosa / metabolism
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Quorum Sensing / drug effects*
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Ultraviolet Rays
Substances
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Bacterial Proteins
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LasR protein, Pseudomonas aeruginosa
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Molecular Probes
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Trans-Activators
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Diazomethane
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Carboxylic Ester Hydrolases
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N-acyl homoserine lactonase