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Virus Res. 2013 Sep;176(1-2):83-90. doi: 10.1016/j.virusres.2013.05.004. Epub 2013 May 20.

Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein.

Author information

1
School of Veterinary Medicine, The University of Zambia, Great East Road Campus, Lusaka, Zambia.

Abstract

Filoviruses (viruses in the genus Ebolavirus and Marburgvirus in the family Filoviridae) cause severe haemorrhagic fever in humans and nonhuman primates. Rapid, highly sensitive, and reliable filovirus-specific assays are required for diagnostics and outbreak control. Characterisation of antigenic sites in viral proteins can aid in the development of viral antigen detection assays such immunochromatography-based rapid diagnosis. We generated a panel of mouse monoclonal antibodies (mAbs) to the nucleoprotein (NP) of Ebola virus belonging to the species Zaire ebolavirus. The mAbs were divided into seven groups based on the profiles of their specificity and cross-reactivity to other species in the Ebolavirus genus. Using synthetic peptides corresponding to the Ebola virus NP sequence, the mAb binding sites were mapped to seven antigenic regions in the C-terminal half of the NP, including two highly conserved regions among all five Ebolavirus species currently known. Furthermore, we successfully produced species-specific rabbit antisera to synthetic peptides predicted to represent unique filovirus B-cell epitopes. Our data provide useful information for the development of Ebola virus antigen detection assays.

KEYWORDS:

Antibody epitope; BDBV; Bundibugyo virus; EBOV; Ebola virus; GP; MARV; Marburg virus; Monoclonal antibody; NP; Nucleoprotein; RAVV; RESTV; Ravn virus; Reston virus; SUDV; Sudan virus; Synthetic peptide; TAFV; Taï Forest virus; VLP; VP; glycoprotein; mAb; monoclonal antibodies; nucleoprotein; viral protein; virus-like particle

PMID:
23702199
PMCID:
PMC3787873
DOI:
10.1016/j.virusres.2013.05.004
[Indexed for MEDLINE]
Free PMC Article

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