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PLoS One. 2013 May 21;8(5):e64023. doi: 10.1371/journal.pone.0064023. Print 2013.

Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.

Author information

1
Confocal and 2-Photon Microscopy Core Facility, Max-Delbrueck-Center for Molecular Medicine Berlin, Berlin, Germany.

Abstract

This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.

PMID:
23700448
PMCID:
PMC3660314
DOI:
10.1371/journal.pone.0064023
[Indexed for MEDLINE]
Free PMC Article

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