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Bioorg Med Chem. 2013 Oct 15;21(20):6186-90. doi: 10.1016/j.bmc.2013.04.062. Epub 2013 May 1.

A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid).

Author information

1
Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5320 Odense M, Denmark.

Abstract

A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.

KEYWORDS:

Cell imaging; Nucleic acid detection; Pyrene excimer; Quencher-free molecular beacon; Unlocked nucleic acid (UNA)

PMID:
23693070
DOI:
10.1016/j.bmc.2013.04.062
[Indexed for MEDLINE]
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