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Emerg Infect Dis. 2013 May;19(5):736-42B. doi: 10.3201/eid1905.130057.

Full-genome deep sequencing and phylogenetic analysis of novel human betacoronavirus.

Author information

1
Wellcome Trust Sanger Institute, Hinxton, UK.

Abstract

A novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient's sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events.

KEYWORDS:

computer-aided design; coronavirus infections; disease transmission; evolution; high-throughput nucleotide sequencing; infectious; molecular; respiratory tract infections; viruses; zoonoses

PMID:
23693015
PMCID:
PMC3647518
DOI:
10.3201/eid1905.130057
[Indexed for MEDLINE]
Free PMC Article
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