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Cell Biol Int. 2013 Oct;37(10):1106-13. doi: 10.1002/cbin.10136. Epub 2013 Jun 5.

C3G overexpression promotes the survival of rat-derived H9C2 cardiomyocytes by p-ERK1/2.

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  • 1Division of Cardiology, Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, China.

Abstract

Integrin β1 subunit and its downstream molecules, such as integrin-linked kinase and focal adhesion kinase, are imperative for promotion of cell proliferation, survival and anti-apoptosis in cardiomyocytes by activation of their downstream pro-survival signalling molecules, such as the phosphorylated extracellular signal-regulated kinase1/2 (p-ERK1/2). As a component of the integrin pathway, C3G (Crk-SH3 domain guanine nucleotide exchange factor) protein may be involved in the promotion of cell proliferation and survival and anti-apoptosis in the H9C2 cardiomyocytes. Rat-derived H9C2 cardiomyocytes were transfected with pCXN2-flag-hC3G, a human C3G overexpression eukaryotic recombinant plasmid. Apoptosis, cell proliferation and survival were analysed in the H9C2 cardiomyocytes either treated with hypoxia/reoxygenation (H/R). Human C3G mRNA overexpression significantly elevated C3G protein expression in H9C2 cardiomyocytes whether treated with H/R or not. C3G overexpression promoted proliferation and survival and anti-apoptosis, and attenuated the proliferative and survival inhibition, and apoptosis induced by H/R by activation of its downstream pro-survival signalling molecule, p-ERK1/2. The results suggest that C3G acts as a pro-survival molecule in H9C2 cardiomyocytes by activation of p-ERK1/2.

KEYWORDS:

Crk-SH3 domain guanine nucleotide exchange factor (C3G); H9C2; apoptosis; cardiomyocyte; cell proliferation; phosphorylated extracellular signal-regulated kinase1/2 (p-ERK1/2)

PMID:
23686869
DOI:
10.1002/cbin.10136
[PubMed - indexed for MEDLINE]
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