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Anal Biochem. 2013 Sep 1;440(1):9-11. doi: 10.1016/j.ab.2013.04.027. Epub 2013 May 17.

A transfection reporter for the prevention of false-negative results in molecular beacon experiments.

Author information

1
Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.

Abstract

We previously developed a molecular beacon-type probe to detect the strand scission in cellular base excision repair and found that the phosphodiester linkages in the fluorophore/quencher linkers were cleaved. This reaction was applied to a transfection reporter, which contained the unmodified phosphodiester in the linker to another type of fluorophore. After cotransfection of cells with the probe and the reporter, the signals were used to detect the incision and to confirm the proper transfection, respectively. This method will contribute to the prevention of false-negative results in experiments using molecular beacon-type probes.

KEYWORDS:

Base excision repair; Molecular beacon; Phosphorothioate; Thymine glycol

PMID:
23685051
DOI:
10.1016/j.ab.2013.04.027
[Indexed for MEDLINE]

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