Format

Send to

Choose Destination
See comment in PubMed Commons below
Cell Host Microbe. 2013 May 15;13(5):602-12. doi: 10.1016/j.chom.2013.04.008.

The Mtb proteome library: a resource of assays to quantify the complete proteome of Mycobacterium tuberculosis.

Author information

1
Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland.

Abstract

Research advancing our understanding of Mycobacterium tuberculosis (Mtb) biology and complex host-Mtb interactions requires consistent and precise quantitative measurements of Mtb proteins. We describe the generation and validation of a compendium of assays to quantify 97% of the 4,012 annotated Mtb proteins by the targeted mass spectrometric method selected reaction monitoring (SRM). Furthermore, we estimate the absolute abundance for 55% of all Mtb proteins, revealing a dynamic range within the Mtb proteome of over four orders of magnitude, and identify previously unannotated proteins. As an example of the assay library utility, we monitored the entire Mtb dormancy survival regulon (DosR), which is linked to anaerobic survival and Mtb persistence, and show its dynamic protein-level regulation during hypoxia. In conclusion, we present a publicly available research resource that supports the sensitive, precise, and reproducible quantification of virtually any Mtb protein by a robust and widely accessible mass spectrometric method.

PMID:
23684311
PMCID:
PMC3766585
DOI:
10.1016/j.chom.2013.04.008
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center